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European Photon & NeutronScience Campus
TRUC
ESRFEuropean Synchrotron Radiation Facility
ILL Institut Laue-Langevin
PSB Partnership for Structural Biology
EMBLEuropean Molecular Biology Laboratory
IBSInstitut de Biologie Structurale
ILL, 2015
PSCMPartnership for Soft Condensed Matter
ILL
EMBL
PSB
PSCM
ESRF
IBS

PSCM Equipment

ALV CGS-3 Static & Dynamic Light Scattering

The ALV CGS-3 Compact Goniometer System (ALV GmbH, Langen, FRG) allows for a simultaneous measurement of static and dynamic light scattering. The temperature can be set between 5°C and 50°C.

Static light scattering (SLS) measures the form factor in the angular range of 17° and 152° (equivalent to Qmax ≈ 0.00255 Å-1, depending on solvent index of refraction).

Combining these techniques yields a supplementary information, the structure sensitive parameter ρ (ρ = RG/RH). It allows for an (overall) shape characterization of particles (e. g. sphere, rod, coil etc.).


Rheometer Anton Paar Physica MCR 501

The Anton Paar Physica MCR 501 rheometer allows on line (SANS and reflectometry beam line) and off line rheological measurements. Plate plate, cone and plate, and Couette geometries are available allowing a wide range of sample viscosity to be studied.


Rheometer Haake Mars II, Thermo Fischer Scientific

This instrument allows the rheological characterization of the flow behaviour and viscoelastic properties of complex fluids and bulk soft matter systems. Six different measuring geometries are available in cone-plate, plate-plate and Couette configuration. Two Peltier controllers allow sample temperature regulation during the measurement in the range from 5 °C up to 85 °C with 0.1°C precision.

  • Instrument parameters
    • Angular resolution: 12 nrad
    • Minimum torque in rotation: Mmin 0.05 mN.m
    • Minimum torque in oscillation: Mmin 0.25 mN.m
    • Maximum torque: Mmax 200 mN.m

  • Tools parameters

    • Couette: Rotor diameter: 25 mm; Gap: 2.126 mm; Sample Volume: 16.1 mL
    • Plate/Plate(Diameter ∅ in mm / angle): 60/0° 20/0°
    • Cone-Plate(Diameter ∅ in mm / angle): 20/2° 20/4° 60/1°

Beaglehole Picometer Light Ellipsometer

A Beaglehole Picometer Light ellipsometer is optimised for interfacial characterisation at the air/liquid and air/solid interfaces. The interfacial excess can be determined to a precision and sensitivity of < 5% of a surfactant monolayer, and with complementary information or optical modelling the data can be used to quantify the adsorbed amount of material at an interface. Data can be acquired at a rate of up to 200 Hz, hence the instrument provides access to the study of fast adsorption kinetics.

The ellipsometer offers two principal benefits to  users. First, it can be used as a pre-screening tool to characterise the kinetics and homogeneity of samples before neutron reflectometry (NR) beam time on FIGARO. Also, the interfacial excess of surface-active material may be compared precisely with respect to a changing variable such as solution composition or temperature. Second, the technique has greater sensitivity to species with a similar scattering length density to that of air (e.g. hydrogenated polymers). Therefore the acquisition of an "optical contrast" during NR measurements can reduce the need for as many NR isotopic contrasts thus saving beam time and deuterated chemicals. Kinetic information may be quantified to complement further the neutron reflectometry data, and inhomogeneity in free liquid surfaces can be identified prior to the modelling of NR data.


Brewster Angle Microscope Nanofilm EP3

The BAM utilizes the fact that when p-polarized light is guided towards an air-water interface, no reflection occurs at a certain incident angle. This angle, the Brewster angle, is determined by Snell's law and depends on the refractive indices of the materials in the system. The Brewster angle for the air-water interface is 53°, and under this condition the image of a pure water surface appears black. Addition of material to the air-water interface modifies the local refractive index (RI), and hence, a small amount of light is reflected and displayed within the image. The image displayed contains areas of varying brightness determined by the particular molecules and packing densities across the sampling area.

Lateral resolution ≥1µm


Contact Angle Measuring Instrument DS114, Kruss

The drops are generated with a reproducibility of 0.3 microliter and placed on the sample at the touch of a finger. Software-controlled contact angle measurement can start immediately after drop placement - without any further actions being required. With image recording rates of up to 61fps even rapid changes in the contact angle can be recorded.


Differential Scanning Calorimeter DSC-131 and DCS-III by Setaram

DSC-131
Crucibles designed to ensure good thermal transfer between the sample and the sensor – Alumina, Aluminum (30 and 100 µl).
T range = -120 to 700°C, atmospheric pressure, air or inert gas
Programmable temperature scanning rate (heating and cooling): 0.1 to 100°C/min.

μDSC-III
The Micro DSCIII has a very low detection limit: calorimetric signals of less than one Microwatt can be detected. The cells are made of Hastelloy C, have a volume of approximately 1 cm3 and are readily removed and easily cleaned.
Temperature range = 5 to 90°C, atmospheric pressure
Programmable temperature scanning rate (heating and cooling): 0.001 to 1.2°C/min.

 


Ellipsometer for Solids

This is an "home made" ellipsometer by Patrice Ballet at the University Joseph Fourier (Grenoble).

The principle of this rotating quarter wave plate ellipsometer can be found at this address :
http://pagesperso-orange.fr/aime.vareille/pages/ellipsometrie/

The laser diodes 2 & 3 are required to find the number of periods of the signal.

The signal acquisition is done with an A/D conversion card at 100 kHz and with a resolution of 16 bits.

The angle of incidence is set to silicon (75 °) but can be changed between 0 and 90 degrees.


HPLC Pump for Lipid Extraction


Plasma Cleaner, Harrick

The Plasma Cleaner serves as an excellent tool for surface cleaning, surface preparation and surface modification. Plasma treatment may be applied to a wide variety of materials, including metals, ceramics, composites, plastics, polymers and biomaterials.


Tensiometer, Kruss K11

Fully automatic determination of surface and interfacial tension for routine measurements in laboratory and quality control applications.

Measuring methods:

  • Ring method (Du Noüy; correction acc. To Huh&Mason, Harkins&Jordan; Zuidema&Waters)
  • Plate method (Wilhelmy)
  • Temperature range: 5 to 90°C
  • Measuring range: SFT / IFT 1 to 999 mN/m, Density: 1 to 2200 kg/m3

Langmuir Trough NIMA 1212D

Nima Langmuir trough 1212D used for the preparation of layers on solid substrates from insoluble amphiphilic molecules, typically lipids but also polymers and proteins.

  • Trough surface 680x200 mm2
  • 2 independent barriers
  • Dipper and well at center
  • Dimensions well: 90x110 mm2 85mm deep
  • Temperature control
  • Pressure sensor uses a Wilhelmy plate (chromatography paper of wet perimeter 20.6mm weight 80mg/m2)
  • Manual and automatic system for Langmuir-Schaefer deposition optimized for 80x50 mm2 silicon substrates

Langmuir Trough NIMA 611

Nima 611 Langmuir trough used for the preparation of layers on solid substrates from insoluble amphiphilic molecules, typically lipids but also polymers and proteins.

  • Trough surface 300x200 mm²
  • 1 barrier
  • Dipper and well at one end
  • Dimensions well: 63x180 mm² 70mm deep
  • Temperature control
  • Pressure sensor uses a Wilhelmy plate (chromatography paper of wet perimeter 20.6mm weight 80mg/m²)
  • Manual system for Langmuir-Schaefer deposition optimized for 50mm wide squared substrates

Langmuir trough NIMA 721BAM

Nima Langmuir trough 712BAM used for the preparation of molecular layers on water surfaces from insoluble amphiphilic molecules, typically lipids but also polymers and proteins. Such layers can also be transferred to solid substrates by the built-in accessory allowing Langmuir-Blodgett deposition.

Langmuir trough characteristics:

  • Maximum area: 716 cm2
  • Minimum area: 30 cm2
  • Barrier speed max: 654.2 cm2.min-1
  • Barrier speed min: 5.2cm2.min-1
  • Dimension well (Area-Deep): 50x20mm2-30mm

Transfer arm type D1L:

  • Amplitude: 25 mm
  • Speed: Min 0.5 - Max 44.5 mm.min-1

Malvern Zeta-sizer

The Zetasizer Nano Z is the perfect system for measuring zeta potential and electrophoretic mobility of colloids and nanoparticles, when the measurement of size or molecular weight is not required.
Laser Doppler Micro-electrophoresis is used to measure zeta potential. An electric field is applied to a solution of molecules or a dispersion of particles, which then move with a velocity related to their zeta potential. This velocity is measured using a patented laser interferometric technique called M3-PALS (Phase analysis Light Scattering). This enables the calculation of electrophoretic mobility, and from this, the zeta potential and zeta potential distribution.

Minimum sample volume: 150 μl
Temperature control range: 0°C - 90°C (+/-0.5%)
Light Source: He-Ne laser 633nm, Max 5mW.
Laser safety: Class 1
Temperature: 15°C – 40°C
Humidity: 35% - 80% non-condensing

SOURCE www.malvern.com


Portable Raman Spectrometer, Ocean Optics

A portable Raman spectrometer (Ocean Optics QE65000) is under commissioning. This spectrometer is equipped with a fiber-optics probe head (Ocean Optics RIP-RPB-785-FC) with a 7.5mm focal length which can be used for in-situ applications. The instrument provides a relatively low resolution and no microscopy option, but due to its light weight, compact size and sturdy design can be used both in the soft-matter laboratory as well as on neutron and x-ray instruments.
The several meters fiber extension allows for large distances between the spectrometer and the sample. The fiber optics probe head can also be combined with a standard cuvette holder for liquid samples. The 785nm laser source is equipped with a safety interlock which can be adapted to the individual experiment setups. The acquisition software (Ocean Optics SpectraSuite) operates on a Windows (XP or higher) PC with USB port.


E4 Quartz Crystal Microbalance, Q-Sense

The QCM is a mass sensing device with the ability to measure very small masses changes on a quartz crystal resonator in real time. It is possible to measure mass changes as small as a fraction of monolayer or single layer of atoms. As the second generation of Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) from Q-Sense, the E4 offers the opportunity to study molecular interactions and molecular adsorption to many different types of surfaces. Applications include proteins, lipids, polyelectrolytes, polymers and cells/bacteria interacting with surfaces or with previously bound molecular layers.

The instrument determines the mass of very thin surface bound layers and simultaneously gives information about their structural (viscoelastic) properties. It is based on the patented QCM-D technique, an extremely sensitive and fast technique providing multi-frequency and dissipation data that are needed to fully understand the state of molecular layers bound to the sensor surface.

  • Number of sensors 4, also possible to measure using only 1, 2 or 3 sensors
  • Volume above each sensor ~ 40 µl using Q-Sense Flow Module for 5 MHz crystals
  • Minimum sample volume ~ 200 µl
  • Working temperature 18 to 45 °C, controlled via the software, stability ± 0.02 K
  • Flow rates 0-1 ml/min
  • Sensor crystals 5 MHz, 14 mm diameter, polished, AT-cut, gold electrodes

Spin Coater Delta6 SUSS MicroTec

Delta6 RC Spin Coater from SUSS MicroTec used to coat solid substrates with polymer or lipid films.

Adaptors available for squared and round substrates of 50mm diameter (or 50x50mm² surface) or rectangular (80x50mm² surface).

Chuck rotation 0-10,000 rpm depending on size (please, check instructions before use).


UV-VIS V-630 Spectrophotometer, Jasco

Characteristics:

  • V-630 Jasco
  • Double-beam spectrophotometer with single monochromator
  • Silicon photodiode detectors
  • Range 190 to 1100 nm
  • Fixed bandpass of 1.5 nm
  • High-speed scanning up to 8,000 nm/min
  • Temperature range: 20 to 70°C

UV-Visible Nanodrop Spectrophotometer

The Nanodrop is a compact, stand-alone UV-Visible spectrophotometer for micro volume analysis of purified nucleic acids and wide variety of proteins.

The sample retention system enables the measurement of highly concentrated samples without need for dilutions.

  • Minimum Sample Volume 1 μL
  • Limit of Detection dsDNA

    • Pedestal: 2.0 ng/μL
    • Standard Cuvette:  0.2 ng/μL


  • Limit of Detection BSA (IgG)

    • Pedestal: 0.06 (0.03) mg/mL
    • Standard Cuvette:  0.006 (0.003) mg/mL

  • Maximum Concentration

    • dsDNA Pedestal:    27,500 ng/μL
    • BSA (IgG) Pedestal:  820 (400) mg/mL



  • Wavelength

    • Range     190 - 850 nm
    • Accuracy  ∓1 nm


  • Pathlength 0.030 to 1.0 mm auto-ranging

AFM-Cypher S Asylum Research

Cypher S

Thanks to its built-in features, the Cypher-S provides significant easiness of use and performance benefits for imaging topography, mechanical, electrical, and magnetic properties in air and liquids.

Instrument Features and proprieties:

  • Standard Scanning modes:

    • Contact : uses feedback on deflection.
    • AC: uses feedback on amplitude.
    • Force: force curve acquisition and mapping in contact or AC mode.

  • SpotOn™: automated laser alignment for an easier positioning of the laser spot on your cantilever.

  • Laser spot size: 3μm enable high-speed AC imaging with ultra-small cantilevers. *

  • Scan size XY: 30μm

  • BlueDrive photo-thermal excitation for AC imaging.

  • Integrated enclosure: allows for 0.1° thermal control and 20dB acoustic isolation.

 


* In general, using ultra-small cantilevers, you can record images in liquids at the speed of one second per image on biological molecules fixed on mica.


Produces an almost ideal cantilever response in liquids by directly exciting photother

mally the cantilever.


AFM-MFP3D Asylum Research

The MFP-3D is ideal for many applications including physics, material science, polymer science, chemistry, nanolithography, bioscience, and quantitative nanoscale measurements.
Thanks to the relatively large XY scanning size, the Z range and the possibility to scan area on sample more than 5mm thick, it is suited to study cells and large samples.

Instrument proprieties:

  • Standard scanning modes:

    • Contact: uses feedback on deflection.
    • AC: uses feedback on amplitude, Piezo actuator mechanical excitation
    • Force:force curve acquisition and mapping

  • Scan size XY: 90 μm

  • Scan range Z: 15 μm


This AFM can be equipped with a cell for liquid imaging (Closed Fluid Cell CCELL) , which offer the opportunity for example to image cells in their growth medium.

Proprieties: Closed Fluid Cell

  • Completely sealed cell
  • Gases and liquids flow trough
  • Maximum pressure: 2.5 psi (17.2 kPa)
  • Sample size: 25 mm; 2 mm thickness

AFM-Veeco Dimension 3100

Beeing logic and built in a simple geometry, it is able to do measurements in samples that are heavy and more than 15mm thick.

Still, it is an instrument capable of imaging specimens with a vertical resolution down to a fraction of a nanometer. The instrument works by measuring the deflection produced by a sharp tip on micron-sized cantilever as it scans across the surface of the specimen. Sample sizes that can be handled by the instrument range from small pieces to 150 mm diameter wafers.

The measurements can be handled at the solely solid/gas interface with this instrument.


Brewster Angle Microscope (BAM) EP3

The Accurion EP3-BAM is used to image thin films on solid or liquid substrates. To obtain image contrast, the BAM uses the principle that at a certain incidence (Brewster) angle the p-polarized laser beam is not reflected at all by the substrate-air interface. The Brewster angle depends only on the refractive index (RI) of the substrate and is equal to 53° in the case of a water substrate. At this incidence angle, nano-thick molecular and/or nanoparticle films that are spread at the water surface will modify locally the RI value, thus yielding a non-zero reflection coefficient and providing image contrast.


Instrument parameters:

  • Light source: laser unit λ 532nm
  • Imaging system CCD camera 768 x 572px
  • Imaging optics:

    • 10X CF Plan Nikon Objective: NA/0.21; Working distance/20.3mm
    • 20X CF Plan Nikon Objective: NA/0.21; Working distance/20.3mm


  • Motorized goniometer:

    • Angle of incidence (AOI) range: 40-90°
    • Angle resolution: 0.001°
    • Absolute angle accuracy: 0.01°


Microscope Olympus BX61

Thanks to the 3 different light sources and the filters and analysers mounted, the BX61 is a very versatile microscope. It allows the users to perform optical observation using the following imaging modes:

  • Bright field (epi or dia-illumination)
  • Phase contrast (dia-illumination)
  • Differential Interference Contrast (epi-illumination)
  • Fluorescence observation (epi-illumination at 3 different wavelengths: UV 360-370nm; Blue 470-490nm and Green 530-550nm).

The objectives mounted on the computer-controlled turret are described in the following table:

Serie                 Magnification   Numerical Aperture   Working Distance (mm)

MPLFLN-10            10                         0.30                                11.0

MPLFLN-20            20                         0.45                                3.1

MPLFLN-50            50                         0.80                                1.0

MPLFLN-100          100                        0.90                                1.0

UPLFLN-10PH         10                         0.30                                10.0

UPLFLN-40PH         40                         0.75                                0.5



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